Validation Data Gallery
Tested Applications
Positive WB detected in | HUVEC cells |
Positive IP detected in | HUVEC cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
12355-1-AP targets ESM1 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ESM1 fusion protein Ag3019 相同性解析による交差性が予測される生物種 |
Full Name | endothelial cell-specific molecule 1 |
Calculated molecular weight | 184 aa, 20 kDa |
Observed molecular weight | 20 kDa |
GenBank accession number | BC011989 |
Gene Symbol | ESM1 |
Gene ID (NCBI) | 11082 |
RRID | AB_3669137 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NQ30 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.
Protocols
Product Specific Protocols | |
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WB protocol for ESM1 antibody 12355-1-AP | Download protocol |
IP protocol for ESM1 antibody 12355-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |