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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using ESM1 Polyclonal antibody (12355-1-AP)

    WB analysis of HUVEC using 12355-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 12355-1-AP (ESM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HUVEC cells using ESM1 Polyclonal antibody (12355-1-AP)

    IP experiment of HUVEC using 12355-1-AP

    IP result of anti-ESM1 (IP:12355-1-AP, 4ug; Detection:12355-1-AP 1:300) with HUVEC cells lysate 640 ug.

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ESM1 Polyclonal antibody

ESM1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12355-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

ESM-1, Endothelial cell-specific molecule 1, Endothelial cell specific molecule 1, endothelial cell molecule 1, endocan

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells
Positive IP detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12355-1-AP targets ESM1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESM1 fusion protein Ag3019 相同性解析による交差性が予測される生物種
Full Name endothelial cell-specific molecule 1
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight20 kDa
GenBank accession numberBC011989
Gene Symbol ESM1
Gene ID (NCBI) 11082
RRIDAB_3669137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.

Protocols

Product Specific Protocols
WB protocol for ESM1 antibody 12355-1-APDownload protocol
IP protocol for ESM1 antibody 12355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols