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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESM1 Polyclonal antibody (32079-1-AP)

    WB analysis using 32079-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 32079-1-AP (ESM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ESM1 Polyclonal antibody

ESM1 Polyclonal Antibody for WB, ELISA
Cat No. 32079-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32079-1-AP targets ESM1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESM1 fusion protein Ag36144 相同性解析による交差性が予測される生物種
Full Name endothelial cell-specific molecule 1
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight20 kDa
GenBank accession numberBC011989
Gene Symbol ESM1
Gene ID (NCBI) 11082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NQ30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ESM1, also known as endothelial cell-specific molecule 1 or endocan, is a protein-coding gene that encodes a secreted protein. It is primarily expressed in endothelial cells of human lung and kidney tissues, and its expression is regulated by cytokines, suggesting a role in endothelium-dependent pathological disorders. ESM1 has been implicated in various diseases, including gastric cancer, hypertension, and cervical cancer. In cervical cancer, ESM1 is overexpressed and acts as an independent prognostic factor associated with poor clinical outcomes. It promotes carcinoma angiogenesis and cervical squamous cell carcinoma progression through the VEGF/ERK signaling pathway.

Protocols

Product Specific Protocols
WB protocol for ESM1 antibody 32079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols