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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ESPN Polyclonal antibody

ESPN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20717-1-AP

Synonyms

LP2654, Espin, DFNB36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESPN Polyclonal antibody (20717-1-AP)

    WB analysis using 20717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20717-1-AP (ESPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using ESPN Polyclonal antibody (20717-1-AP)

    WB analysis of Neuro-2a using 20717-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20717-1-AP (ESPN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ESPN Polyclonal antibody (20717-1-AP)

    IHC staining of human ovary cancer using 20717-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20717-1-AP (ESPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue, Neuro-2a cells, rat testis
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20717-1-AP targets ESPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name espin
Calculated molecular weight 92 kDa
Observed molecular weight 30-33 kDa, 110 kDa
GenBank accession numberNM_031475
Gene symbol ESPN
Gene ID (NCBI) 83715
RRIDAB_10858327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ESPN, also named as DFNB36, is a multifunctional actin-bundling protein. ESPN plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells. Defects in ESPN are the cause of deafness autosomal recessive type 36 (DFNB36). Defects in ESPN are the cause of deafness autosomal dominant without vestibular involvement (DFNAWVI). The antibody is specific to ESPN.

Protocols

Product Specific Protocols
WB protocol for ESPN antibody 20717-1-APDownload protocol
IHC protocol for ESPN antibody 20717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols