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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ESR2 Monoclonal antibody (60197-1-Ig)

    WB analysis of MCF-7 using 60197-1-Ig (same clone as 60197-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW 1990 cells using ESR2 Monoclonal antibody (60197-1-Ig)

    WB analysis of SW 1990 using 60197-1-Ig (same clone as 60197-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ESR2 Monoclonal antibody (60197-1-Ig)

    WB analysis of human brain using 60197-1-Ig (same clone as 60197-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ESR2 Monoclonal antibody, PBS Only

ESR2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60197-1-PBS
Clone No.5D2C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60197-1-PBS targets ESR2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESR2 fusion protein Ag5103 相同性解析による交差性が予測される生物種
Full Name estrogen receptor 2 (ER beta)
Calculated molecular weight 59 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC024181
Gene Symbol ESR2
Gene ID (NCBI) 2100
RRIDAB_10897162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92731
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).