ESR2 Recombinant antibody
ESR2 Recombinant Antibody for IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF, ELISA
Conjugate
Unconjugated
CloneNo.
240412F2
Cat no : 83447-3-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 83447-3-RR is ESR2 Fusion Protein expressed in E. coli.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ESR2 fusion protein Ag5103 相同性解析による交差性が予測される生物種 |
| Full Name | estrogen receptor 2 (ER beta) |
| Calculated molecular weight | 59 kDa |
| GenBank accession number | BC024181 |
| Gene symbol | ESR2 |
| Gene ID (NCBI) | 2100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ESR2 antibody 83447-3-RR | Download protocol |
| FC protocol for ESR2 antibody 83447-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
