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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ESR2 Beta 3 Polyclonal antibody, PBS Only

ESR2 Beta 3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55441-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human endometrial cancer using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human endometrial cancer using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human breast cancer using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human breast cancer using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IF Staining of HeLa using 55441-1-AP (same clone as 55441-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 3 antibody (55441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red) DAPI (blue). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55441-1-PBS targets ESR2 Beta 3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name estrogen receptor 2 (ER beta)
Calculated molecular weight 58 kDa
GenBank accession numberNM_001437
Gene symbol ESR2
Gene ID (NCBI) 2100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual