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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ESR2 Beta 6 Polyclonal antibody

ESR2 Beta 6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55471-1-AP

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 6, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    WB analysis of mouse spleen using 55471-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    WB analysis of mouse spleen using 55471-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    WB analysis of mouse spleen using 55471-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    IHC staining of human prostate cancer using 55471-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    IHC staining of human cervix using 55471-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    IHC staining of human cervix using 55471-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    IHC staining of human prostate cancer using 55471-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    IHC staining of human prostate cancer using 55471-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ESR2 Beta 6 Polyclonal antibody (55471-1-AP)

    IF Staining of HeLa using 55471-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 6 antibody (55471-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red), DAPI (Blue).

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Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman prostate cancer tissue, human cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55471-1-AP targets ESR2 Beta 6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name estrogen receptor 2 (ER beta)
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_001437
Gene symbol ESR2
Gene ID (NCBI) 2100
RRIDAB_2881344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual

Protocols

Product Specific Protocols
WB protocol for ESR2 Beta 6 antibody 55471-1-APDownload protocol
IHC protocol for ESR2 Beta 6 antibody 55471-1-APDownload protocol
IF protocol for ESR2 Beta 6 antibody 55471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Pharmaceuticals (Basel)

Isoliquiritigenin Inhibits the Growth of Colorectal Cancer Cells through the ESR2/PI3K/AKT Signalling Pathway

Authors - Fenglin Luo
View Article