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  • KD/KO Validated

ESRRB Polyclonal antibody

ESRRB Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22644-1-AP

Synonyms

DFNB35, ERR2, ERRb, ERRbeta, ERRbeta 2, ESRL2, ESRRB, ESRRB protein, estrogen related receptor beta, NR3B2


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Tested Applications

Positive WB detected inPC-13 cells, A549 cells
Positive IP detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22644-1-AP is ESRRB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESRRB fusion protein Ag18523 相同性解析による交差性が予測される生物種
Full Name estrogen-related receptor beta
Calculated molecular weight 508 aa, 56 kDa
Observed molecular weight 48-56 kDa
GenBank accession numberBC131517
Gene symbol ESRRB
Gene ID (NCBI) 2103
RRIDAB_2879140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

Protocols

Product Specific Protocols
WB protocol for ESRRB antibody 22644-1-APDownload protocol
IP protocol for ESRRB antibody 22644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
mouseIF

Nucleic Acids Res

The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity.

Authors - Stephanie Chrysanthou
humanWB,IF

Genes Dev

Role of the small subunit processome in the maintenance of pluripotent stem cells.

Authors - Kwon Tae You
mouseWB,IF

Front Cell Dev Biol

ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.

Authors - Shuai Yu
  • KD Validated
mouseWB

ACS Nano

Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.

Authors - Jie Zhang
humanWB

Nat Commun

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.

Authors - J Mathieu
  • KO Validated