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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ESRRB Monoclonal antibody, PBS Only

ESRRB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E10C1

Cat no : 66818-1-PBS

Synonyms

ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ESRRB Monoclonal antibody (66818-1-Ig)

    WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ESRRB Monoclonal antibody (66818-1-Ig)

    WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ESRRB Monoclonal antibody (66818-1-Ig)

    IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ESRRB Monoclonal antibody (66818-1-Ig)

    IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using ESRRB Monoclonal antibody (66818-1-Ig)

    IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using ESRRB Monoclonal antibody (66818-1-Ig)

    IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66818-1-PBS targets ESRRB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESRRB fusion protein Ag18626 相同性解析による交差性が予測される生物種
Full Name estrogen-related receptor beta
Calculated molecular weight 508 aa, 56 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC131517
Gene symbol ESRRB
Gene ID (NCBI) 2103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.