ETFA Polyclonal antibody

Name: ETFA Polyclonal antibody
Brand: Proteintech
SKU: 12262-1-AP

ETFA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12262-1-AP

Synonyms

MADD, GA2, EMA, Alpha-ETF, Alpha ETF

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 12262-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human ileum using 12262-1-AP
human ileum tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 12262-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12262-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 12262-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12262-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12262-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFA antibody (12262-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).
FC experiment of MCF-7 using 12262-1-AP
1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug ETFA Polyclonal antibody (12262-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 12262-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Y79 cells, human ileum tissue, mouse small intestine tissue, rat small intestine tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IF	See 1 publications below

Product Information

12262-1-AP targets ETFA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ETFA fusion protein Ag2931 相同性解析による交差性が予測される生物種
Full Name	electron-transfer-flavoprotein, alpha polypeptide
Calculated molecular weight	30 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC015526
Gene symbol	ETFA
Gene ID (NCBI)	2108
RRID	AB_2100540
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.

Protocols

Product Specific Protocols
WB protocol for ETFA antibody 12262-1-AP	Download protocol
IHC protocol for ETFA antibody 12262-1-AP	Download protocol
IF protocol for ETFA antibody 12262-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. Authors - Bin Jiang View Article
mouse	WB	Cell Mol Life Sci Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. Authors - Min-Zhi Peng View Article
human	WB	Bioorg Chem Pomalidomide sensitizes lung cancer cells to TRAIL/CDDP-induced apoptosis via directly targeting electron transfer flavoprotein alpha subunit Authors - Liangping Li View Article
human	WB	J Proteome Res Proteomic analysis of nuclei isolated from cancer cell lines treated with indenoisoquinoline NSC 724998, a novel topoisomerase I inhibitor. Authors - Han Bingnan B View Article
human	WB	J Proteomics Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis. Authors - Muto Taika T View Article
human	WB	Proteome Sci Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe. Authors - Singaravelu Ragunath R View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ETFA Polyclonal antibody

ETFA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 12262-1-AP

WB analysis of human ileum using 12262-1-AP

WB analysis of mouse small intestine using 12262-1-AP

WB analysis of Y79 using 12262-1-AP

IHC staining of human breast cancer using 12262-1-AP

IHC staining of human breast cancer using 12262-1-AP

IF Staining of HeLa using 12262-1-AP

FC experiment of MCF-7 using 12262-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Pomalidomide sensitizes lung cancer cells to TRAIL/CDDP-induced apoptosis via directly targeting electron transfer flavoprotein alpha subunit

Proteomic analysis of nuclei isolated from cancer cell lines treated with indenoisoquinoline NSC 724998, a novel topoisomerase I inhibitor.

Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis.

Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe.