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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ETFA Monoclonal antibody, PBS Only

ETFA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12G5

Cat no : 67661-1-PBS

Synonyms

Alpha ETF, EMA, ETFA, GA2, MADD

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ETFA Monoclonal antibody (67661-1-Ig)

    WB analysis of mouse brain using 67661-1-Ig (same clone as 67661-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ETFA Monoclonal antibody (67661-1-Ig)

    WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ETFA Monoclonal antibody (67661-1-Ig)

    WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ETFA Monoclonal antibody (67661-1-Ig)

    IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ETFA Monoclonal antibody (67661-1-Ig)

    IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ETFA Monoclonal antibody (67661-1-Ig)

    IF Staining of HepG2 using 67661-1-Ig (same clone as 67661-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ETFA antibody (67661-1-Ig, Clone: 3C12G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67661-1-PBS targets ETFA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ETFA fusion protein Ag30257 相同性解析による交差性が予測される生物種
Full Name electron-transfer-flavoprotein, alpha polypeptide
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC015526
Gene symbol ETFA
Gene ID (NCBI) 2108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)