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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ETS1 Monoclonal antibody (66598-1-Ig)

    WB analysis of HepG2 using 66598-1-Ig (same clone as 66598-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ETS1 Monoclonal antibody (66598-1-Ig)

    WB analysis of Jurkat using 66598-1-Ig (same clone as 66598-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using ETS1 Monoclonal antibody (66598-1-Ig)

    WB analysis of Daudi using 66598-1-Ig (same clone as 66598-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ETS1 Monoclonal antibody (66598-1-Ig)

    WB analysis of HSC-T6 using 66598-1-Ig (same clone as 66598-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of mouse lung using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of mouse lung using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of mouse small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of mouse small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of rat lung using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of rat lung using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of rat small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using ETS1 Monoclonal antibody (66598-1-Ig)

    IHC staining of rat small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ETS1 Monoclonal antibody (66598-1-Ig)

    IF Staining of A431 using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ETS1 Monoclonal antibody, PBS Only

ETS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66598-1-PBS
Clone No.4F2D4

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein C-ets-1, EWSR2, ETS 1, 4F2D4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66598-1-PBS targets ETS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ETS1 fusion protein Ag2127 相同性解析による交差性が予測される生物種
Full Name v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Calculated molecular weight 441 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC017314
Gene Symbol ETS1
Gene ID (NCBI) 2113
RRIDAB_2881958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14921
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.