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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ETS2 Recombinant antibody (85113-4-RR)

    IF Staining of HeLa using 85113-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ETS2 Recombinant antibody (85113-4-RR)

    IF Staining of MCF-7 using 85113-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using ETS2 Recombinant antibody (85113-4-RR)

    FC experiment of MCF-7 using 85113-4-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ETS2 Recombinant antibody (85113-4-RR, Clone:242817H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85113-4-RR

    Affinity and Kinetic Characterization of 85113-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85113-4-RR against Human ETS2 were performed. The affinity constant is below 1 pM.

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ETS2 Recombinant antibody

ETS2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85113-4-RR
Clone No.242817H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

242817H8, ETS-2, ETS2IT1, Protein C ets 2, Protein C-ets-2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85113-4-RR targets ETS2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ETS2 fusion protein Ag2929 相同性解析による交差性が予測される生物種
Full Name v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
Calculated molecular weight 469 aa, 53 kDa
GenBank accession numberBC017040
Gene Symbol ETS2
Gene ID (NCBI) 2114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15036
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for ETS2 antibody 85113-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols