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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ETV4 Recombinant antibody (83891-6-RR)

    WB analysis of K-562 using 83891-6-RR (same clone as 83891-6-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 83891-6-RR (ETV4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ETV4 Recombinant antibody (83891-6-RR)

    IHC staining of human colon cancer using 83891-6-RR (same clone as 83891-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83891-6-RR (ETV4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ETV4 Recombinant antibody (83891-6-RR)

    IHC staining of human stomach cancer using 83891-6-RR (same clone as 83891-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83891-6-RR (ETV4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ETV4 Recombinant antibody (83891-6-RR)

    IF Staining of A431 using 83891-6-RR (same clone as 83891-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETV4 antibody (83891-6-RR, Clone: 241042E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using ETV4 Recombinant antibody (83891-6-RR)

    FC experiment of U2OS using 83891-6-RR (same clone as 83891-6-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ETV4 Recombinant antibody (83891-6-RR, Clone:241042E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83891-6-RR

    Affinity and Kinetic Characterization of 83891-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83891-6-RR against Human ETV4 were performed. The affinity constant is 0.157 nM.

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ETV4 Recombinant antibody, PBS Only

ETV4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83891-6-PBS
Clone No.241042E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PEA3, ETS translocation variant 4, E1A-F, E1AF, Adenovirus E1A enhancer-binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83891-6-PBS targets ETV4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ETV4 fusion protein Ag0984 相同性解析による交差性が予測される生物種
Full Name ets variant 4
Calculated molecular weight 54 kDa
Observed molecular weight 61-65 kDa
GenBank accession numberBC007242
Gene Symbol ETV4
Gene ID (NCBI) 2118
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP43268
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.