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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ETV5 Monoclonal antibody, PBS Only (Capture)

ETV5 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4A4

Cat no : 66657-1-PBS

Synonyms

ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ETV5 Monoclonal antibody (66657-1-Ig)

    WB analysis using 66657-1-Ig (same clone as 66657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66657-1-Ig (ETV5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66657-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50024-1

    Sandwich ELISA standard curve of MP50024-1

    Sandwich ELISA standard curve ofMP50024-1, ETV5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66657-1-PBS. Detection antibody: HRP-conjugated 66657-2-PBS. Standard: Ag3708. Range: 0.313-20 ng/m.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66657-1-PBS targets ETV5 as part of a matched antibody pair:

MP50024-1: 66657-1-PBS capture and 66657-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ETV5 fusion protein Ag3708 相同性解析による交差性が予測される生物種
Full Name ets variant 5
Calculated molecular weight 510 aa, 58 kDa
Observed molecular weight 58-60 kDa
GenBank accession numberBC007333
Gene symbol ETV5
Gene ID (NCBI) 2119
RRIDAB_2882014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney