Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13182-1-AP targets ETV6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ETV6 fusion protein Ag3852 相同性解析による交差性が予測される生物種 |
| Full Name | ets variant 6 |
| Calculated molecular weight | 452 aa, 53 kDa |
| Observed molecular weight | 57-65 kDa |
| GenBank accession number | BC043399 |
| Gene Symbol | ETV6 |
| Gene ID (NCBI) | 2120 |
| RRID | AB_2246688 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41212 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ETV6, the E26 transformation-specific (ETS) family transcription repressor and tumor suppressor variant 6 gene, resides on chromosome 12p13, encodes a 57 kDa ETS family transcription factor with 3 highly conserved functional domains(PMID: 32841218). ETV6 is a well-studied hematopoietic transcription factor reported to play a key functional role in hematopoiesis, megakaryopoiesis, cell growth, and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ETV6 antibody 13182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |