Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55367-1-AP targets EVC2 in WB, IHC, ELISA applications and shows reactivity with Mouse, Human samples.
| Tested Reactivity | Mouse, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Ellis van Creveld syndrome 2 |
| Calculated molecular weight | 148 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_147127 |
| Gene Symbol | EVC2 |
| Gene ID (NCBI) | 132884 |
| RRID | AB_3086445 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UK5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EVC2 (also known as LIMBIN) is close proximity in divergent orientation to EVC and mutations in both human genes lead to the chondrodysplasia Ellis-van Creveld (EvC) syndrome. Typical symptoms of EvC include shorter limbs and ribs, postaxial polydactyly, dysplastic nails, and cardiovascular defects (PMID: 28081373). Additionally, EvC syndrome has been characterized as a ciliopathy because the EVC and EVC2 proteins are intracellularly localized at the bottom of the primary cilium, where they form a heterotrimeric protein complex with SMO and transduce Hedgehog(Hh) signaling (PMID: 30410447). EVC2 is expressed in the heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. EVC2 has 3 isoforms with the molecular mass of 140-148 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EVC2 antibody 55367-1-AP | Download protocol |
| IHC protocol for EVC2 antibody 55367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |