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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EVI1 Polyclonal antibody

EVI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23201-1-AP

Synonyms

MECOM, MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using EVI1 Polyclonal antibody (23201-1-AP)

    WB analysis of SKOV-3 using 23201-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using EVI1 Polyclonal antibody (23201-1-AP)

    WB analysis of SKOV-3 using 23201-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using EVI1 Polyclonal antibody (23201-1-AP)

    IHC staining of human thyroid cancer using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using EVI1 Polyclonal antibody (23201-1-AP)

    IHC staining of human thyroid cancer using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using EVI1 Polyclonal antibody (23201-1-AP)

    IHC staining of human urothelial carcinoma using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using EVI1 Polyclonal antibody (23201-1-AP)

    IHC staining of human urothelial carcinoma using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using EVI1 Polyclonal antibody (23201-1-AP)

    IF Staining of SKOV-3 using 23201-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using EVI1 antibody (23201-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23201-1-AP targets EVI1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EVI1 fusion protein Ag19603 相同性解析による交差性が予測される生物種
Full Name ecotropic viral integration site 1
Calculated molecular weight 1239 aa, 139 kDa
Observed molecular weight 118 kDa
GenBank accession numberBC130520
Gene symbol EVI1
Gene ID (NCBI) 2122
RRIDAB_2879230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EVI1 antibody 23201-1-APDownload protocol
IHC protocol for EVI1 antibody 23201-1-APDownload protocol
IF protocol for EVI1 antibody 23201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Sci Adv

Predicting master transcription factors from pan-cancer expression data.

Authors - Jessica Reddy
View Article
humanCoIP

Oncol Rep

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis

Authors - Zhitao Chen
View Article