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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using EVI2A Polyclonal antibody (17415-1-AP)

    WB analysis of human brain using 17415-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17415-1-AP (EVI2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using EVI2A Polyclonal antibody (17415-1-AP)

    WB analysis of human brain using 17415-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17415-1-AP (EVI2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using EVI2A Polyclonal antibody (17415-1-AP)

    IHC staining of human brain using 17415-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17415-1-AP (EVI2A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using EVI2A Polyclonal antibody (17415-1-AP)

    IHC staining of human brain using 17415-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17415-1-AP (EVI2A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EVI2A Polyclonal antibody (17415-1-AP)

    IF Staining of HepG2 using 17415-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EVI2A antibody (17415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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EVI2A Polyclonal antibody

EVI2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17415-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EVI-2A, EVI2, EVI 2A, EVDA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17415-1-AP targets EVI2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EVI2A fusion protein Ag11075 相同性解析による交差性が予測される生物種
Full Name ecotropic viral integration site 2A
Calculated molecular weight 236 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC035572
Gene Symbol EVI2A
Gene ID (NCBI) 2123
RRIDAB_2101143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22794
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EVI2A antibody 17415-1-APDownload protocol
IHC protocol for EVI2A antibody 17415-1-APDownload protocol
IF protocol for EVI2A antibody 17415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

A combined omics approach to generate the surface atlas of human naive CD4+ T cells during early TCR activation.

Authors - Anke Graessel
View Article
humanWB

Oncol Res

Integrative bioinformatics and in vitro exploration of EVI2A expression: unraveling its immunological and prognostic implications in kidney renal clear cell carcinoma

Authors - Rong Liu
View Article