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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EVI5 Recombinant antibody (84738-2-RR)

    IF Staining of U2OS using 84738-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EVI5 antibody (84738-2-RR, Clone: 241918D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84738-2-RR

    Affinity and Kinetic Characterization of 84738-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84738-2-RR against Human EVI5 were performed. The affinity constant is 1.11 nM.

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EVI5 Recombinant antibody

EVI5 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84738-2-RR
Clone No.241918D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

NB4S, 241918D11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84738-2-RR targets EVI5 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EVI5 fusion protein Ag26739 相同性解析による交差性が予測される生物種
Full Name ecotropic viral integration site 5
Calculated molecular weight 810 aa, 93 kDa
GenBank accession numberBC156793
Gene Symbol EVI5
Gene ID (NCBI) 7813
RRIDAB_3672122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EVI5 functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. It may play a role in cytokinesis (PMID: 16439210).

Protocols

Product Specific Protocols
IF protocol for EVI5 antibody 84738-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols