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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EVL Polyclonal antibody

EVL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13484-1-AP

Synonyms

Ena/VASP like protein, Enah/Vasp like, EVL, RNB6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using EVL Polyclonal antibody (13484-1-AP)

    WB analysis of Jurkat using 13484-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13484-1-AP (EVL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human small intestine using 13484-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human small intestine using 13484-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human lung using 13484-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using EVL Polyclonal antibody (13484-1-AP)

    IHC staining of human lung using 13484-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman small intestine tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13484-1-AP targets EVL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EVL fusion protein Ag4302 相同性解析による交差性が予測される生物種
Full Name Enah/Vasp-like
Calculated molecular weight 418 aa, 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC032358
Gene symbol EVL
Gene ID (NCBI) 51466
RRIDAB_10644121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EVL (Ena/VASP-like protein) is implicated in processes that requires dynamic actin remodeling such as axon guidance and platelet activation.It has two isoforms, EVL and EVL-I,that are highly expressed in hematopoietic cells of thymus and spleen,and both isoforms can be phosphorylated by PKA in vitro(PMID10945997).

Protocols

Product Specific Protocols
WB protocol for EVL antibody 13484-1-APDownload protocol
IHC protocol for EVL antibody 13484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pers Med

Immunocyte Infiltration Analysis and Immunohistochemistry Identify EVL as a Potential Prognostic Biomarker for Pancreatic Cancer

Authors - Yan Du
View Article
mouseIHC

Clin Transl Med

Genetic and pharmacological inhibition of METTL3 alleviates renal fibrosis by reducing EVL m6A modification through an IGF2BP2-dependent mechanism

Authors - Wei-Jian Ni
View Article