Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, mouse testis tissue, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
55191-1-AP targets EWS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Ewing sarcoma breakpoint region 1 |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 68-70 kDa |
| GenBank accession number | NM_005243 |
| Gene Symbol | EWS |
| Gene ID (NCBI) | 2130 |
| RRID | AB_10860907 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01844 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EWS antibody 55191-1-AP | Download protocol |
| IHC protocol for EWS antibody 55191-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Nucleic Acids Res The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells. | ||
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
Mol Ther Nucleic Acids Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. | ||
J Reprod Dev Lipid droplets synthesized during luteinization are degraded after pregnancy |