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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXD1 Polyclonal antibody

EXD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17313-1-AP

Synonyms

piRNA biogenesis protein EXD1, Inactive exonuclease EXD1, Exonuclease 3'-5' domain-like-containing protein 1, Exonuclease 3'-5' domain-containing protein 1, EXDL1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EXD1 Polyclonal antibody (17313-1-AP)

    WB analysis of HeLa using 17313-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using EXD1 Polyclonal antibody (17313-1-AP)

    WB analysis of SH-SY5Y using 17313-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using EXD1 Polyclonal antibody (17313-1-AP)

    IHC staining of human testis using 17313-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using EXD1 Polyclonal antibody (17313-1-AP)

    IHC staining of human testis using 17313-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EXD1 Polyclonal antibody (17313-1-AP)

    IF Staining of HeLa using 17313-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXD1 antibody (17313-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17313-1-AP targets EXD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXD1 fusion protein Ag11282 相同性解析による交差性が予測される生物種
Full Name exonuclease 3'-5' domain containing 1
Calculated molecular weight 514 aa, 58 kDa
Observed molecular weight 36 kDa, 58 kDa, 75 kDa
GenBank accession numberBC030628
Gene symbol EXD1
Gene ID (NCBI) 161829
RRIDAB_2878379
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXD1, also named as EXDL1, has three isoforms with MW 58 kDa, 36 kDa and 65 kDa. This antibody can recognize all these three isoforms.

Protocols

Product Specific Protocols
WB protocol for EXD1 antibody 17313-1-APDownload protocol
IHC protocol for EXD1 antibody 17313-1-APDownload protocol
IF protocol for EXD1 antibody 17313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Andrology

Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes

Authors - Opeyemi Olotu
View Article