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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXO1 Polyclonal antibody (16253-1-AP)

    WB analysis using 16253-1-AP (same clone as 16253-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using EXO1 Polyclonal antibody (16253-1-AP)

    WB analysis of Jurkat using 16253-1-AP (same clone as 16253-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16253-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse thymus tissue using EXO1 Polyclonal antibody (16253-1-AP)

    IP experiment of mouse thymus using 16253-1-AP (same clone as 16253-1-PBS)

    IP result of anti-EXO1 (IP:16253-1-AP, 4ug; Detection:16253-1-AP 1:500) with mouse thymus tissue lysate 2800ug. This data was developed using the same antibody clone with 16253-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EXO1 Polyclonal antibody, PBS Only

EXO1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 16253-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16253-1-PBS targets EXO1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXO1 fusion protein Ag9253 相同性解析による交差性が予測される生物種
Full Name exonuclease 1
Calculated molecular weight 94 kDa
Observed molecular weight 115-120 kDa
GenBank accession numberBC007491
Gene Symbol EXO1
Gene ID (NCBI) 9156
RRIDAB_2278140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ84
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.