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KD/KO Validated

SEC6 Polyclonal antibody

Name: SEC6 Polyclonal antibody
Brand: Proteintech
SKU: 14703-1-AP

SEC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14703-1-AP

Synonyms

EXOC3, SEC6L1, Exocyst complex component Sec6, Exocyst complex component 3

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of human brain using 14703-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14703-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14703-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 14703-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 14703-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14703-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14703-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 14703-1-AP
Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14703-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue, COLO 320 cells, HeLa cells, HepG2 cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

14703-1-AP targets SEC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SEC6 fusion protein Ag6383 相同性解析による交差性が予測される生物種
Full Name	exocyst complex component 3
Calculated molecular weight	87 kDa
Observed molecular weight	86 kDa
GenBank accession number	BC064569
Gene symbol	SEC6
Gene ID (NCBI)	11336
RRID	AB_2101542
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

Protocols

Product Specific Protocols
WB protocol for SEC6 antibody 14703-1-AP	Download protocol
IHC protocol for SEC6 antibody 14703-1-AP	Download protocol
IF protocol for SEC6 antibody 14703-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Microbiol Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells. Authors - Hiroki Takeuchi KD Validated View Article
human	WB	Cell Microbiol Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway. Authors - Takeuchi Hiroki H KD Validated View Article
rat	WB	Dev Biol Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition. Authors - Youssef G G View Article
human	IF,WB,IHC	Pathol Res Pract Exosome secretion related gene signature predicts chemoresistance in patients with colorectal cancer Authors - Liang Ming View Article
human	WB	Nat Commun Salmonella exploits membrane reservoirs for invasion of host cells Authors - Hongxian Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC6 Polyclonal antibody

SEC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of human brain using 14703-1-AP

WB analysis of HeLa using 14703-1-AP

WB analysis of HepG2 using 14703-1-AP

WB analysis of COLO 320 using 14703-1-AP

IHC staining of human gliomas using 14703-1-AP

IHC staining of human gliomas using 14703-1-AP

IHC staining of human gliomas using 14703-1-AP

IF Staining of HepG2 using 14703-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells.

Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway.

Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition.

Exosome secretion related gene signature predicts chemoresistance in patients with colorectal cancer

Salmonella exploits membrane reservoirs for invasion of host cells