Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 11913-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11913-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 11913-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 11913-1-AP
IP result of anti-Sec8 (IP:11913-1-AP, 4ug; Detection:11913-1-AP 1:300) with Jurkat cells lysate 2800ug.
IHC staining of human colon cancer using 11913-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11913-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11913-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Sec8 Polyclonal antibody

Sec8 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11913-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

SEC8L1, REC8, KIAA1699, Exocyst complex component Sec8, Exocyst complex component 4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse testis tissue, U-251 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

11913-1-AP targets Sec8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Sec8 fusion protein Ag2514 相同性解析による交差性が予測される生物種
Full Name	exocyst complex component 4
Calculated molecular weight	110 kDa
Observed molecular weight	110-120 kDa
GenBank accession number	BC026174
Gene Symbol	Sec8
Gene ID (NCBI)	60412
RRID	AB_2101565
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96A65
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Sec8 antibody 11913-1-AP	Download protocol
IHC protocol for Sec8 antibody 11913-1-AP	Download protocol
IP protocol for Sec8 antibody 11913-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. Authors - Anish Vaibhav Sharda View Article
mouse	WB	Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis Authors - Kunli Zhao View Article
human	WB	Biol Res REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells. Authors - Miao Liu View Article
mouse	WB	Commun Biol The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium Authors - S M Niedziółka View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 11913-1-AP

WB analysis of mouse testis using 11913-1-AP

WB analysis of U-251 using 11913-1-AP

IP experiment of Jurkat using 11913-1-AP

IHC staining of human colon cancer using 11913-1-AP

IHC staining of human colon cancer using 11913-1-AP

IHC staining of human colon cancer using 11913-1-AP

Sec8 Polyclonal antibody

Sec8 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells.

The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium