Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, human adrenal gland tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
17593-1-AP targets EXOC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EXOC5 fusion protein Ag11524 相同性解析による交差性が予測される生物種 |
| Full Name | exocyst complex component 5 |
| Calculated molecular weight | 708 aa, 82 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | BC041126 |
| Gene Symbol | EXOC5 |
| Gene ID (NCBI) | 10640 |
| RRID | AB_2101582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00471 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EXOC5 (SEC10), a component of the exocyst complex, is a 77-kDa protein with a broad tissue distribution (PubMed: 9119050). The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| IHC protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| IF protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| IP protocol for EXOC5 antibody 17593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly.
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Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis | ||
Mol Cancer Res EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.
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Cells The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells | ||
J Biol Chem Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model.
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