Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, HEK-293 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 5 publications below |
| CoIP | See 2 publications below |
Product Information
12014-1-AP targets Exo70 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Exo70 fusion protein Ag2628 相同性解析による交差性が予測される生物種 |
| Full Name | exocyst complex component 7 |
| Calculated molecular weight | 735 aa, 83 kDa |
| Observed molecular weight | 74 kDa |
| GenBank accession number | BC011045 |
| Gene Symbol | Exo70 |
| Gene ID (NCBI) | 23265 |
| RRID | AB_2101698 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPT5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Exo70 antibody 12014-1-AP | Download protocol |
| IHC protocol for Exo70 antibody 12014-1-AP | Download protocol |
| IP protocol for Exo70 antibody 12014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. | ||
Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis | ||
Mol Neurobiol The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons. | ||
Life Sci Alliance An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation |