Exo70 Polyclonal antibody

Name: Exo70 Polyclonal antibody
Brand: Proteintech
SKU: 12014-1-AP

Exo70 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12014-1-AP

Synonyms

2 5 3p, EX070, Exo70, Exo70p, EXOC1, EXOC7, exocyst complex component 7, KIAA1067, YJL085W

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of human brain using 12014-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12014-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12014-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12014-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12014-1-AP
IP result of anti-Exo70 (IP:12014-1-AP, 3ug; Detection:12014-1-AP 1:600) with mouse brain tissue lysate 2640ug.
IHC staining of human gliomas using 12014-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 12014-1-AP (Exo70 antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 12014-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue, HEK-293 cells, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 4 publications below
CoIP	See 2 publications below

Product Information

12014-1-AP targets Exo70 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Exo70 fusion protein Ag2628 相同性解析による交差性が予測される生物種
Full Name	exocyst complex component 7
Calculated molecular weight	735 aa, 83 kDa
Observed molecular weight	74 kDa
GenBank accession number	BC011045
Gene symbol	Exo70
Gene ID (NCBI)	23265
RRID	AB_2101698
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Exo70 antibody 12014-1-AP	Download protocol
IHC protocol for Exo70 antibody 12014-1-AP	Download protocol
IP protocol for Exo70 antibody 12014-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. Authors - Anish Vaibhav Sharda View Article
mouse	WB	Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis Authors - Kunli Zhao View Article
rat	IF	Mol Neurobiol The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons. Authors - Matías Lira View Article
human	WB,IF	Placenta Exocyst complex protein expression in the human placenta. Authors - I M Gonzalez View Article
mouse	WB,IF,CoIP	Biol Res Exo70 intracellular redistribution after repeated mild traumatic brain injury. Authors - Matías Lira View Article
human,rat	IF,CoIP	Life Sci Alliance An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation Authors - Priyadarshini Ravindran View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Exo70 Polyclonal antibody

Exo70 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of human brain using 12014-1-AP

WB analysis of mouse brain using 12014-1-AP

WB analysis of HEK-293 using 12014-1-AP

WB analysis of human brain using 12014-1-AP

IP experiment of mouse brain using 12014-1-AP

IHC staining of human gliomas using 12014-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.

Exocyst complex protein expression in the human placenta.

Exo70 intracellular redistribution after repeated mild traumatic brain injury.

An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation