Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of SGC-7901 using 12585-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12585-1-AP
WB result of EXOSC1 antibody (12585-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC1 transfected MCF-7 cells.
WB analysis using 12585-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 12585-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 12585-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12585-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12585-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12585-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12585-1-AP
IP result of anti-EXOSC1 (IP:12585-1-AP, 4ug; Detection:12585-1-AP 1:600) with HeLa cells lysate 3000 ug.
IHC staining of human lymphoma using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 12585-1-AP (EXOSC1 antibody) at dilution of 1:100 (under 10x lens).

Featured Product
KD/KO Validated

EXOSC1 Polyclonal antibody

EXOSC1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12585-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

hCsl4p, Exosome component 1, Exosome complex component CSL4, Csl4p, CSL4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SGC-7901 cells, HeLa cells, human stomach tissue, Jurkat cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

12585-1-AP targets EXOSC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, yeast
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EXOSC1 fusion protein Ag3286 相同性解析による交差性が予測される生物種
Full Name	exosome component 1
Calculated molecular weight	195 aa, 21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC022067
Gene Symbol	EXOSC1
Gene ID (NCBI)	51013
RRID	AB_2101714
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3B2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

Protocols

Product Specific Protocols
WB protocol for EXOSC1 antibody 12585-1-AP	Download protocol
IHC protocol for EXOSC1 antibody 12585-1-AP	Download protocol
IP protocol for EXOSC1 antibody 12585-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
yeast	WB	Am J Med Genet A Pontocerebellar hypoplasia associated with p.Arg183Trp homozygous variant in EXOSC1 gene: A case report Authors - Nadirah S Damseh View Article
human	WB	Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication Authors - Tobias Moll View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of SGC-7901 using 12585-1-AP

WB analysis of MCF-7 using 12585-1-AP

WB analysis using 12585-1-AP

WB analysis using 12585-1-AP

WB analysis of human stomach using 12585-1-AP

WB analysis of HeLa using 12585-1-AP

WB analysis of Jurkat using 12585-1-AP