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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXOSC1 Recombinant antibody

EXOSC1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240938C6

Cat no : 83875-5-RR

Synonyms

Exosome component 1, Exosome complex component CSL4, Csl4p, CSL4, CGI-108

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC1 Recombinant antibody (83875-5-RR)

    WB analysis using 83875-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83875-5-RR (EXOSC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83875-5-RR

    Affinity and Kinetic Characterization of 83875-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83875-5-RR against Human EXOSC1 were performed. The affinity constant is 1.86 nM.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, SGC-7901 cells, MCF-7 cells, HEK-293T cells, rat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83875-5-RR is EXOSC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EXOSC1 fusion protein Ag3286 相同性解析による交差性が予測される生物種
Full Name exosome component 1
Calculated molecular weight 195 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC022067
Gene symbol EXOSC1
Gene ID (NCBI) 51013
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

Protocols

Product Specific Protocols
WB protocol for EXOSC1 antibody 83875-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols