☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11178-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Rrp6p, RRP6, PM/Scl-100, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11178-1-AP targets EXOSC10 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC10 fusion protein Ag1666 相同性解析による交差性が予測される生物種
Full Name exosome component 10
Calculated molecular weight 98 kDa
Observed molecular weight100 kDa
GenBank accession numberBC039901
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2293792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01780
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Protocols

Product Specific Protocols
WB protocol for EXOSC10 antibody 11178-1-APDownload protocol
IHC protocol for EXOSC10 antibody 11178-1-APDownload protocol
IF protocol for EXOSC10 antibody 11178-1-APDownload protocol
IP protocol for EXOSC10 antibody 11178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
humanWB

Cell Rep

Human Telomerase RNA Processing and Quality Control.

Authors - Chi-Kang Tseng
  • KD Validated
mouseWB,RIP

Development

Post-transcription regulation by the exosome complex is required for cell survival and forebrain development by repressing P53 signaling.

Authors - Pauline Antonie Ulmke
  • KO Validated
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
humanWB

Cancer Res

NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer

Authors - Ruihui Xie
Loading...