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EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16731-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16731-1-AP targets EXOSC10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC10 fusion protein Ag10189 相同性解析による交差性が予測される生物種
Full Name exosome component 10
Calculated molecular weight 885 aa, 101 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC073788
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2101730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01780
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Protocols

Product Specific Protocols
WB protocol for EXOSC10 antibody 16731-1-APDownload protocol
IHC protocol for EXOSC10 antibody 16731-1-APDownload protocol
IF protocol for EXOSC10 antibody 16731-1-APDownload protocol
IP protocol for EXOSC10 antibody 16731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

PeerJ

EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification

Authors - Zhi-Yong Meng
  • KD Validated
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