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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXOSC10 Monoclonal antibody

EXOSC10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1D6G9

Cat no : 68315-1-Ig

Synonyms

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC10 Monoclonal antibody (68315-1-Ig)

    WB analysis using 68315-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68315-1-Ig (EXOSC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Immunoprecipitation (IP) experiment of HeLa cells using EXOSC10 Monoclonal antibody (68315-1-Ig)

    IP experiment of HeLa using 68315-1-Ig

    IP result of anti-EXOSC10 (IP:68315-1-Ig, 4ug; Detection:68315-1-Ig 1:1000) with HeLa cells lysate 1720 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EXOSC10 Monoclonal antibody (68315-1-Ig)

    IF Staining of HeLa using 68315-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXOSC10 antibody (68315-1-Ig, Clone: 1D6G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68315-1-Ig targets EXOSC10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EXOSC10 fusion protein Ag28587 相同性解析による交差性が予測される生物種
Full Name exosome component 10
Calculated molecular weight 98 kDa
Observed molecular weight98 kDa
GenBank accession numberBC039901
Gene symbol EXOSC10
Gene ID (NCBI) 5394
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Protocols

Product Specific Protocols
WB protocol for EXOSC10 antibody 68315-1-IgDownload protocol
IF protocol for EXOSC10 antibody 68315-1-IgDownload protocol
IP protocol for EXOSC10 antibody 68315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols