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EXOSC2 Polyclonal antibody

Name: EXOSC2 Polyclonal antibody
Brand: Proteintech
SKU: 14805-1-AP
Rating: 5.00 (2 reviews)

EXOSC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 14805-1-AP

Synonyms

Rrp4p, RRP4, Ribosomal RNA-processing protein 4, hRrp4p, Exosome component 2

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 14805-1-AP
WB result of EXOSC2 antibody (14805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC2 transfected HEK-293 cells.
WB analysis of HeLa using 14805-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14805-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14805-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14805-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14805-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14805-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14805-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14805-1-AP
IP result of anti-EXOSC2 (IP:14805-1-AP, 4ug; Detection:14805-1-AP 1:1000) with HeLa cells lysate 1080ug.
IHC staining of human skin cancer using 14805-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin cancer using 14805-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of MCF-7 using 14805-1-AP
Immunofluorescent analysis of MCF-7 cells, using EXOSC2 antibody 14805-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 14805-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, HEK-293 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 1 publications below

Product Information

14805-1-AP targets EXOSC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EXOSC2 fusion protein Ag6525 相同性解析による交差性が予測される生物種
Full Name	exosome component 2
Calculated molecular weight	33 kDa
Observed molecular weight	30-33 kDa
GenBank accession number	BC000747
Gene symbol	EXOSC2
Gene ID (NCBI)	23404
RRID	AB_2101837
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Protocols

Product Specific Protocols
WB protocol for EXOSC2 antibody 14805-1-AP	Download protocol
IHC protocol for EXOSC2 antibody 14805-1-AP	Download protocol
IF protocol for EXOSC2 antibody 14805-1-AP	Download protocol
IP protocol for EXOSC2 antibody 14805-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Cell Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression. Authors - Hani Goodarzi View Article
human		Cell The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest. Authors - Jakob Trendel View Article
human	WB	Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication Authors - Tobias Moll View Article
human	WB	Mol Biotechnol EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal Authors - Chen-Guang Lv KD Validated View Article
human,mouse	WB	Mol Cell The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex Authors - Dimitrios Papadopoulos View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Tsimafei (Verified Customer) (12-04-2022)	50µg of total protein were loaded on a gel. Incubation ON at 4C Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: mESCs EXOSC2 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:14805-1-AP)
FH Nikolaus (Verified Customer) (07-14-2022)	Antibodies were incubated ON at 4C. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mESCs EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:14805-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXOSC2 Polyclonal antibody

EXOSC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 14805-1-AP

WB analysis of HeLa using 14805-1-AP

WB analysis of HepG2 using 14805-1-AP

WB analysis of Jurkat using 14805-1-AP

WB analysis of HeLa using 14805-1-AP

WB analysis of MCF-7 using 14805-1-AP

WB analysis of HeLa using 14805-1-AP

WB analysis of HepG2 using 14805-1-AP

IP experiment of HeLa using 14805-1-AP

IHC staining of human skin cancer using 14805-1-AP

IHC staining of human skin cancer using 14805-1-AP

IF Staining of MCF-7 using 14805-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal

The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex

Reviews