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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC2 Monoclonal antibody (66099-1-Ig)

    WB analysis using 66099-1-Ig (same clone as 66099-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EXOSC2 Monoclonal antibody (66099-1-Ig)

    WB analysis of HeLa using 66099-1-Ig (same clone as 66099-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EXOSC2 Monoclonal antibody (66099-1-Ig)

    WB analysis of HEK-293 using 66099-1-Ig (same clone as 66099-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EXOSC2 Monoclonal antibody (66099-1-Ig)

    WB analysis of HepG2 using 66099-1-Ig (same clone as 66099-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using EXOSC2 Monoclonal antibody (66099-1-Ig)

    IHC staining of human liver using 66099-1-Ig (same clone as 66099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using EXOSC2 Monoclonal antibody (66099-1-Ig)

    IHC staining of human skin cancer using 66099-1-Ig (same clone as 66099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using EXOSC2 Monoclonal antibody (66099-1-Ig)

    IHC staining of human skin cancer using 66099-1-Ig (same clone as 66099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EXOSC2 Monoclonal antibody (66099-1-Ig)

    IF Staining of HepG2 using 66099-1-Ig (same clone as 66099-1-PBS)

    Immunofluorescent analysis of () fixed HepG2 cells using 66099-1-Ig (EXOSC2 antibody) at dilution of 1:25. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

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EXOSC2 Monoclonal antibody, PBS Only

EXOSC2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66099-1-PBS
Clone No.1G8B1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66099-1-PBS targets EXOSC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EXOSC2 fusion protein Ag7003 相同性解析による交差性が予測される生物種
Full Name exosome component 2
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000747
Gene Symbol EXOSC2
Gene ID (NCBI) 23404
RRIDAB_2881498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13868
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].