Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of MCF-7 using 25292-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 25292-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25292-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 25292-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 25292-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25292-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25292-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25292-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 25292-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 25292-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 25292-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 25292-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 25292-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 25292-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC7 antibody (25292-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EXOSC7 Polyclonal antibody

EXOSC7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25292-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Ribosomal RNA-processing protein 42, KIAA0116, hRrp42p, Exosome component 7, Exosome complex component RRP42

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, A431 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected in	human colon tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

25292-1-AP targets EXOSC7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EXOSC7 fusion protein Ag18212 相同性解析による交差性が予測される生物種
Full Name	exosome component 7
Calculated molecular weight	291 aa, 32 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC012831
Gene Symbol	EXOSC7
Gene ID (NCBI)	23016
RRID	AB_2880011
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15024
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA

Protocols

Product Specific Protocols
WB protocol for EXOSC7 antibody 25292-1-AP	Download protocol
IHC protocol for EXOSC7 antibody 25292-1-AP	Download protocol
IF protocol for EXOSC7 antibody 25292-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication Authors - Tobias Moll View Article
human	WB,IF,IP	Cell Rep ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells Authors - M Carmen Lafita-Navarro View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 25292-1-AP

WB analysis of K-562 using 25292-1-AP

WB analysis of HepG2 using 25292-1-AP

WB analysis of A431 using 25292-1-AP

WB analysis of COLO 320 using 25292-1-AP

WB analysis of HEK-293 using 25292-1-AP

WB analysis of Jurkat using 25292-1-AP

WB analysis of HeLa using 25292-1-AP

IHC staining of human kidney using 25292-1-AP

IHC staining of human colon using 25292-1-AP

IHC staining of human kidney using 25292-1-AP

IHC staining of human kidney using 25292-1-AP

IHC staining of human colon using 25292-1-AP

IF Staining of HeLa using 25292-1-AP

EXOSC7 Polyclonal antibody

EXOSC7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells