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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC7 Monoclonal antibody (68456-1-Ig)

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EXOSC7 Monoclonal antibody (68456-1-Ig)

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EXOSC7 Monoclonal antibody (68456-1-Ig)

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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EXOSC7 Monoclonal antibody

EXOSC7 Monoclonal Antibody for WB, ELISA
Cat No. 68456-1-Ig
Clone No.2A4C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Synonyms

KIAA0116, hRrp42p, Exosome component 7, Exosome complex component RRP42, EAP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSaos-2 cells, HSC-T6 cells, PANC-1 cells, NIH/3T3 cells, 4TA cells, HT-29 cells, LNCaP cells, MDA-MB-231 cells, IMR-32 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, pig brain tissue, Caco-2 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68456-1-Ig targets EXOSC7 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EXOSC7 fusion protein Ag18322 相同性解析による交差性が予測される生物種
Full Name exosome component 7
Calculated molecular weight 291 aa, 32 kDa
Observed molecular weight35 kDa
GenBank accession numberBC012831
Gene Symbol EXOSC7
Gene ID (NCBI) 23016
RRIDAB_3085168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15024
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA.

Protocols

Product Specific Protocols
WB protocol for EXOSC7 antibody 68456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols