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KD/KO Validated

EXOSC8 Polyclonal antibody

Name: EXOSC8 Polyclonal antibody
Brand: Proteintech
SKU: 11979-1-AP
Rating: 5.00 (2 reviews)

EXOSC8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 11979-1-AP

Synonyms

RRP43, Ribosomal RNA-processing protein 43, p9, Opa-interacting protein 2, OIP2

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 11979-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11979-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11979-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11979-1-AP
IP result of anti-EXOSC8 (IP:11979-1-AP, 4ug; Detection:11979-1-AP 1:500) with HEK-293 cells lysate 1560ug.
IHC staining of human colon using 11979-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11979-1-AP (EXOSC8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11979-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11979-1-AP (EXOSC8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of 4T1 using 11979-1-AP
Immunofluorescent analysis of 4T1 cells, using EXOSC8 antibody 11979-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 11979-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, human brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	4T1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

11979-1-AP targets EXOSC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EXOSC8 fusion protein Ag2605 相同性解析による交差性が予測される生物種
Full Name	exosome component 8
Calculated molecular weight	276 aa, 30 kDa
Observed molecular weight	30-35 kDa
GenBank accession number	BC020773
Gene symbol	EXOSC8
Gene ID (NCBI)	11340
RRID	AB_2101981
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.

Protocols

Product Specific Protocols
WB protocol for EXOSC8 antibody 11979-1-AP	Download protocol
IHC protocol for EXOSC8 antibody 11979-1-AP	Download protocol
IF protocol for EXOSC8 antibody 11979-1-AP	Download protocol
IP protocol for EXOSC8 antibody 11979-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities. Authors - Caitlin A Nichols KO Validated View Article
human	WB	Nat Commun EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia. Authors - Veronika Boczonadi KD Validated View Article
human	WB	PLoS One Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA. Authors - Saeki Makio M View Article
human	WB	Neuromuscul Disord New subtype of PCH1C caused by novel EXOSC8 variants in a 16-year-old Spanish patient. Authors - María Elena Rodríguez-García View Article
human	WB	Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication Authors - Tobias Moll View Article
human	WB	Nature Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance Authors - Lin Shan View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Tsimafei (Verified Customer) (12-04-2022)	50µg of total protein were used. Incubation ON at 4C Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: mESCs EXOSC8 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11979-1-AP)
FH Nikolaus (Verified Customer) (05-24-2022)	Antibodies applied after 2 rounds of stripping, signal still satisfactory Applications: Western Blot Primary Antibody Dilution: 1/2000 Cell Tissue Type: mESC EXOSC8 Antibody Western Blot validation (1/2000 dilution) in mESC (Cat no:11979-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXOSC8 Polyclonal antibody

EXOSC8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 11979-1-AP

WB analysis of HeLa using 11979-1-AP

WB analysis of human brain using 11979-1-AP

IP experiment of HEK-293 using 11979-1-AP

IHC staining of human colon using 11979-1-AP

IHC staining of human colon using 11979-1-AP

IF Staining of 4T1 using 11979-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.

EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.

Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA.

New subtype of PCH1C caused by novel EXOSC8 variants in a 16-year-old Spanish patient.

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance

Reviews