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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC9 Monoclonal antibody (67636-1-Ig)

    WB analysis using 67636-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HeLa cells using EXOSC9 Monoclonal antibody (67636-1-Ig)

    WB analysis of HeLa using 67636-1-Ig

    WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EXOSC9 Monoclonal antibody (67636-1-Ig)

    IHC staining of human breast cancer using 67636-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EXOSC9 Monoclonal antibody (67636-1-Ig)

    IHC staining of mouse kidney using 67636-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EXOSC9 Monoclonal antibody (67636-1-Ig)

    IHC staining of rat kidney using 67636-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EXOSC9 Monoclonal antibody (67636-1-Ig)

    IHC staining of human breast cancer using 67636-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EXOSC9 Monoclonal antibody (67636-1-Ig)

    IF Staining of HeLa using 67636-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

EXOSC9 Monoclonal antibody

EXOSC9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67636-1-Ig
Clone No.3F4G1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, HeLa cells, A549 cells, MCF-7 cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67636-1-Ig targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EXOSC9 fusion protein Ag19783 相同性解析による交差性が予測される生物種
Full Name exosome component 9
Calculated molecular weight 456 aa, 51 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC142978
Gene Symbol EXOSC9
Gene ID (NCBI) 5393
RRIDAB_2882837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06265
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Protocols

Product Specific Protocols
WB protocol for EXOSC9 antibody 67636-1-IgDownload protocol
IHC protocol for EXOSC9 antibody 67636-1-IgDownload protocol
IF protocol for EXOSC9 antibody 67636-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols