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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EXPH5 Polyclonal antibody

EXPH5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22199-1-AP

Synonyms

exophilin 5, EXPH5, KIAA0624, SLAC2 B

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using EXPH5 Polyclonal antibody (22199-1-AP)

    WB analysis of A431 using 22199-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22199-1-AP (EXPH5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using EXPH5 Polyclonal antibody (22199-1-AP)

    IHC staining of human liver using 22199-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22199-1-AP (EXPH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using EXPH5 Polyclonal antibody (22199-1-AP)

    IHC staining of human liver using 22199-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22199-1-AP (EXPH5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22199-1-AP is EXPH5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXPH5 fusion protein Ag17449 相同性解析による交差性が予測される生物種
Full Name exophilin 5
Calculated molecular weight 1989 aa, 223 kDa
Observed molecular weight 222-240 kDa
GenBank accession numberBC113119
Gene symbol EXPH5
Gene ID (NCBI) 23086
RRIDAB_2879026
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXPH5, also named as KIAA0624 or SLAC2B, is a 1989 amino acid protein, which contains one RabBD (Rab-binding) domain. EXPH5 is expressed in keratinocytes. EXPH5 may act as Rab effector protein and play a role in vesicle trafficking. EXPH5 plays a role in maintaining keratinocyte integrity, and in inherited skin fragility.

Protocols

Product Specific Protocols
WB protocol for EXPH5 antibody 22199-1-APDownload protocol
IHC protocol for EXPH5 antibody 22199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Genes Dev

CALML5 is a ZNF750- and TINCR-induced protein that binds stratifin to regulate epidermal differentiation.

Authors - Bryan K Sun
View Article