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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXTL2 Polyclonal antibody (12828-1-AP)

    WB analysis using 12828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12828-1-AP (EXTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EXTL2 Polyclonal antibody (12828-1-AP)

    IHC staining of mouse brain using 12828-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EXTL2 Polyclonal antibody (12828-1-AP)

    IHC staining of mouse brain using 12828-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using EXTL2 Polyclonal antibody (12828-1-AP)

    IHC staining of rat brain using 12828-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using EXTL2 Polyclonal antibody (12828-1-AP)

    IHC staining of rat brain using 12828-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using EXTL2 Polyclonal antibody (12828-1-AP)

    IF Staining of SH-SY5Y using 12828-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EXTL2 antibody (12828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EXTL2 Polyclonal antibody (12828-1-AP)

    IF Staining of HeLa using 12828-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EXTL2 antibody (12828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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EXTL2 Polyclonal antibody

EXTL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12828-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha GalNAcT EXTL2, Alpha-1,4-N-acetylhexosaminyltransferase EXTL2, Alpha-GalNAcT EXTL2, EC:2.4.1.223, exostoses (multiple) like 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12828-1-AP targets EXTL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXTL2 fusion protein Ag3548 相同性解析による交差性が予測される生物種
Full Name exostoses (multiple)-like 2
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight 37-43 kDa
GenBank accession numberBC036015
Gene Symbol EXTL2
Gene ID (NCBI) 2135
RRIDAB_2102130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBQ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Exostosin-like 2 (EXTL2) is one of three EXT-like genes in the human genome that are homologous to EXT1 and EXT2, encodes a transferase that adds not only GlcNAc but also N-acetylgalactosamine to the glycosaminoglycan (GAG)-protein linkage region via an α1,4-linkage. Loss of the enzyme EXTL2, a regulatory mechanism serving to limit CSPG synthesis, resulted in more extensive versican deposition into a lysolecithin-induced demyelinated injury, a larger area of myelin disruption with an associated decrease in axonal density within the lesion, and greater accumulation of macrophages and microglia (PMID: 32703234, 23395820)

Protocols

Product Specific Protocols
WB protocol for EXTL2 antibody 12828-1-APDownload protocol
IHC protocol for EXTL2 antibody 12828-1-APDownload protocol
IF protocol for EXTL2 antibody 12828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols