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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EYA2 Polyclonal antibody

EYA2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30607-1-AP

Synonyms

EC:3.1.3.48, EAB1

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse eye tissue using EYA2 Polyclonal antibody (30607-1-AP)

    IHC staining of mouse eye using 30607-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 30607-1-AP (EYA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using EYA2 Polyclonal antibody (30607-1-AP)

    IHC staining of mouse eye using 30607-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 30607-1-AP (EYA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EYA2 Polyclonal antibody (30607-1-AP)

    IF Staining of HeLa using 30607-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA2 antibody (30607-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30607-1-AP targets EYA2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EYA2 fusion protein Ag31827 相同性解析による交差性が予測される生物種
Full Name eyes absent homolog 2 (Drosophila)
Calculated molecular weight 64 kDa
GenBank accession numberBC013882
Gene symbol EYA2
Gene ID (NCBI) 2139
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. It can be detected two bands of 70 kDa and 56 kDa in the neuroblastoma cells by western blot(PMID:12039049).

Protocols

Product Specific Protocols
IHC protocol for EYA2 antibody 30607-1-APDownload protocol
IF protocol for EYA2 antibody 30607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols