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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EYA4 Polyclonal antibody

EYA4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24691-1-AP

Synonyms

CMD1J, DFNA10, EYA4, Eyes absent homolog 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EYA4 Polyclonal antibody (24691-1-AP)

    WB analysis using 24691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24691-1-AP (EYA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EYA4 Polyclonal antibody (24691-1-AP)

    WB analysis using 24691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24691-1-AP (EYA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EYA4 Polyclonal antibody (24691-1-AP)

    IHC staining of human skeletal muscle using 24691-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EYA4 Polyclonal antibody (24691-1-AP)

    IHC staining of human skeletal muscle using 24691-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EYA4 Polyclonal antibody (24691-1-AP)

    IF Staining of HeLa using 24691-1-AP

    Immunofluorescent analysis of HeLa cells using 24691-1-AP (EYA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24691-1-AP is EYA4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EYA4 fusion protein Ag20466 相同性解析による交差性が予測される生物種
Full Name eyes absent homolog 4 (Drosophila)
Calculated molecular weight 639 aa, 70 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC041063
Gene symbol EYA4
Gene ID (NCBI) 2070
RRIDAB_2879675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EYA4 (Eyes absent homolog 4) participates directly in the modulation of cellular signaling through its phosphatase activity and plays a role in the embryonic and post-natal development of the mature organs of CORTI. This protein may be involved in the development of the eye. It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for EYA4 antibody 24691-1-APDownload protocol
IHC protocol for EYA4 antibody 24691-1-APDownload protocol
IF protocol for EYA4 antibody 24691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Res

Lin28a maintains a subset of adult muscle stem cells in an embryonic-like state

Authors - Peng Wang
View Article