Validation Data Gallery
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Tested Applications
| Positive WB detected in | RAW 264.7 cells, HL-60 cells, HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
20852-1-AP targets EZH1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EZH1 fusion protein Ag14732 相同性解析による交差性が予測される生物種 |
| Full Name | enhancer of zeste homolog 1 (Drosophila) |
| Calculated molecular weight | 747 aa, 85 kDa |
| Observed molecular weight | 80-95 kDa |
| GenBank accession number | BC015882 |
| Gene Symbol | EZH1 |
| Gene ID (NCBI) | 2145 |
| RRID | AB_10863659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92800 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EZH1 antibody 20852-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nat Commun Gain and loss of function variants in EZH1 disrupt neurogenesis and cause dominant and recessive neurodevelopmental disorders | ||
Oxid Med Cell Longev The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome. | ||
Eur J Med Chem Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. | ||
Drug Discov Ther Ezh1 regulates expression of Cpg15/Neuritin in mouse cortical neurons.
| ||
Photochem Photobiol The m6 A reader YTHDC2 regulates UVB-induced DNA damage repair and histone modification |