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KD/KO Validated

EZH2 Polyclonal antibody

Name: EZH2 Polyclonal antibody
Brand: Proteintech
SKU: 21800-1-AP
Rating: 5.00 (1 reviews)

EZH2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 21800-1-AP

Synonyms

KMT6, EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 21800-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 21800-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 21800-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 21800-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21800-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 21800-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 21800-1-AP
Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21800-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21800-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21800-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 21800-1-AP
IP result of anti-EZH2 (IP:21800-1-AP, 4ug; Detection:21800-1-AP 1:1000) with HepG2 cells lysate 3400ug.
IHC staining of human lung squamous cell carcinoma using 21800-1-AP
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 21800-1-AP (EZH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 21800-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (21800-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 21800-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EZH2 (21800-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 21800-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	DU 145 cells, HEK-293 cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, Jurkat cells, A431 cells, HepG2 cells, rat kidney tissue, C6 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human lung squamous cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 21 publications below
WB	See 80 publications below
IF	See 10 publications below
IP	See 3 publications below
CoIP	See 4 publications below
ChIP	See 8 publications below
RIP	See 1 publications below

Product Information

21800-1-AP targets EZH2 in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EZH2 fusion protein Ag16789 相同性解析による交差性が予測される生物種
Full Name	enhancer of zeste homolog 2 (Drosophila)
Calculated molecular weight	751 aa, 86 kDa
Observed molecular weight	90-102 kDa
GenBank accession number	BC010858
Gene symbol	EZH2
Gene ID (NCBI)	2146
RRID	AB_10858790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Protocols

Product Specific Protocols
WB protocol for EZH2 antibody 21800-1-AP	Download protocol
IHC protocol for EZH2 antibody 21800-1-AP	Download protocol
IF protocol for EZH2 antibody 21800-1-AP	Download protocol
IP protocol for EZH2 antibody 21800-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer Authors - Rashmi Bharti View Article
human	WB	Circ Res VE-Cadherin-Mediated Epigenetic Regulation of Endothelial Gene Expression. Authors - Marco F Morini View Article
human	WB,IF	Nat Struct Mol Biol lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal. Authors - Pingping Zhu View Article
		Cancer Res HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc. Authors - Yinghui Li View Article
human,mouse	WB	Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI Authors - Bojun Li KD Validated View Article
human	WB	Nat Commun CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression. Authors - Yongqing Liu KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Nikhil (Verified Customer) (10-16-2019)

very nice

Applications: Western Blot,
Cell Tissue Type: Colon cancer cells

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EZH2 Polyclonal antibody

EZH2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 21800-1-AP

WB analysis of DU 145 using 21800-1-AP

WB analysis of Raji using 21800-1-AP

WB analysis of rat kidney using 21800-1-AP

WB analysis of HEK-293 using 21800-1-AP

WB analysis of mouse kidney using 21800-1-AP

WB analysis using 21800-1-AP

WB analysis of NIH/3T3 using 21800-1-AP

WB analysis of A431 using 21800-1-AP

WB analysis of HepG2 using 21800-1-AP

IP experiment of HepG2 using 21800-1-AP

IHC staining of human lung squamous cell carcinoma using 21800-1-AP

IF Staining of HepG2 using 21800-1-AP

FC experiment of HepG2 using 21800-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

VE-Cadherin-Mediated Epigenetic Regulation of Endothelial Gene Expression.

lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal.

HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.

Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.

Reviews

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