EZH2 Monoclonal antibody, PBS Only

WB result of EZH2 antibody (66476-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EZH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

Various cell lines were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

Jurkat cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

ROS1728 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

4T1 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

Jurkat cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EZH2 (66476-1-Ig, Clone:1F10A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.