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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EZH2 Recombinant antibody, PBS Only (Capture)

EZH2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242309F11

Cat no : 84824-2-PBS

Synonyms

EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EZH2 Recombinant antibody (84824-2-RR)

    WB analysis using 84824-2-RR (same clone as 84824-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84824-2-RR (EZH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84824-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EZH2 Recombinant antibody (84824-2-RR)

    IF Staining of HepG2 using 84824-2-RR (same clone as 84824-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-2-RR, Clone: 242309F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84824-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01605-1

    Cytometric bead array standard curve of MP01605-1

    Cytometric bead array standard curve of MP01605-1, EZH2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84824-2-PBS. Detection antibody: 84824-3-PBS. Standard: Ag16789. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01605-2

    Cytometric bead array standard curve of MP01605-2

    Cytometric bead array standard curve of MP01605-2, EZH2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84824-2-PBS. Detection antibody: 84824-1-PBS. Standard: Ag16789. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84824-2-RR

    Affinity and Kinetic Characterization of 84824-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84824-2-RR against Human EZH2 were performed. The affinity constant is 2.73 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84824-2-PBS targets EZH2 as part of a matched antibody pair:

MP01605-1: 84824-2-PBS capture and 84824-3-PBS detection (validated in Cytometric bead array)

MP01605-2: 84824-2-PBS capture and 84824-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EZH2 fusion protein Ag16789 相同性解析による交差性が予測される生物種
Full Name enhancer of zeste homolog 2 (Drosophila)
Calculated molecular weight 751 aa, 86 kDa
Observed molecular weight 90-102 kDa
GenBank accession numberBC010858
Gene symbol EZH2
Gene ID (NCBI) 2146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)