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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30730-1-AP

Synonyms

CD105, ORW, HHT1, ENG, Endoglin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Endoglin/CD105 Polyclonal antibody (30730-1-AP)

    WB analysis using 30730-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30730-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Endoglin/CD105 Polyclonal antibody (30730-1-AP)

    IHC staining of human renal cell carcinoma using 30730-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 30730-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Endoglin/CD105 Polyclonal antibody (30730-1-AP)

    IHC staining of human renal cell carcinoma using 30730-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 30730-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Endoglin/CD105 Polyclonal antibody (30730-1-AP)

    IHC staining of human placenta using 30730-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30730-1-AP (ENG antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Endoglin/CD105 Polyclonal antibody (30730-1-AP)

    IHC staining of human placenta using 30730-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30730-1-AP (ENG antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Endoglin/CD105 Polyclonal antibody (30730-1-AP)

    IF Staining of human placenta using 30730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Endoglin/CD105 antibody (30730-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Endoglin/CD105 Polyclonal antibody (30730-1-AP)

    IF Staining of human placenta using 30730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Endoglin/CD105 antibody (30730-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHUVEC cells, human placenta tissue
Positive IHC detected inhuman renal cell carcinoma tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30730-1-AP targets Endoglin/CD105 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
Observed molecular weight80-90 kDa
GenBank accession numberBC014271
Gene symbol CD105
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulfide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 30730-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 30730-1-APDownload protocol
IF protocol for Endoglin/CD105 antibody 30730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols