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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Endoglin/CD105 Monoclonal antibody, PBS Only

Endoglin/CD105 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2D5E8

Cat no : 67075-1-PBS

Synonyms

CD105, 2D5E8, HHT1, ENG, Endoglin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    WB analysis using 67075-1-Ig (same clone as 67075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    WB analysis of HL-60 using 67075-1-Ig (same clone as 67075-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    WB analysis of HUVEC using 67075-1-Ig (same clone as 67075-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    WB analysis of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human breast cancer using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human breast cancer using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human renal cell carcinoma using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IHC staining of human renal cell carcinoma using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IF Staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IF Staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IF Staining of human liver cancer using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IF Staining of human liver cancer using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Endoglin/CD105 Monoclonal antibody (67075-1-Ig)

    IF Staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67075-1-PBS targets Endoglin/CD105 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag27837 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC014271
Gene symbol CD105
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)