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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody, PBS Only

Endoglin/CD105 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230047E5

Cat no : 98013-1-PBS

Synonyms

CD105, 230047E5, HHT1, ENG, Endoglin

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Endoglin/CD105 Rabbit Recombinant Antib (98013-1-PBS)

    FC experiment of human PBMCs using 98013-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 (98013-1-PBS, Clone:230047E5) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Endoglin/CD105 Rabbit Recombinant Antib (98013-1-RR)

    FC experiment of human PBMCs using 98013-1-RR (same clone as 98013-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (98013-1-RR, Clone:230047E5) and PE-conjugated Goat Anti-Rabbit IgG(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 98013-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98013-1-RR

    Affinity and Kinetic Characterization of 98013-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98013-1-RR against Human Endoglin/CD105 were performed. The affinity constant is 6.83 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98013-1-PBS targets Endoglin/CD105 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name endoglin
Calculated molecular weight 71 kDa
GenBank accession numberBC014271
Gene symbol CD105
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
FC protocol for Endoglin/CD105 antibody 98013-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols